Journal: Scientific Reports

Article Title: Infection with Mycobacterium tuberculosis induces the Warburg effect in mouse lungs

doi: 10.1038/srep18176

Figure Lengend Snippet: Confocal imaging of HK3, LDHA and ATP6a3 isoform of V-ATPase expression in macrophages and CD3 + cells in mouse lungs ( A–F ). Immunohistochemical staining, confocal imaging, and 3D reconstruction and analysis were carried out using 20 μm lung tissue sections obtained from uninfected control and M. tuberculosis -infected C57BL/6 mice at day 30 post-infection (D30). Tissue sections were stained for nuclei (DAPI, blue); HK3, LDHA, or ATP6a3 isoform of V-ATPase (green); M. tuberculosis (red), CD3 + cells (T lymphocyte, white); and IBA-1 (macrophage, cyan). Bar = 150 μm. Quantitative analysis of HK3, LDHA and ATP6a3 expression by confocal imaging in macrophages and CD3 + cells in lungs of infected and uninfected mice ( G–I ). The expression levels of each target protein under both conditions were obtained by measuring the positive pixels and their intensities from same number of cells of the specific regions of interests (ROIs). Three sections per lung region were examined for each animal and 4 animals per condition were analyzed. Data shown are means of fold change ± SDs of the measurements from four mice at each time point relative to background expressions from each condition. *** indicates p ≤ 0.005.

Article Snippet: Lung tissue sections were analyzed by five-color immunohistochemical staining with antibodies for nuclei: DAPI; M. tuberculosis : biotin antibody: Genetex, Irvine, CA; hexokinase 3 (HK3): Biorbyt, Cambridge, UK; subunit isoform a3 of V0 domain of V-type H + ATPase (ATP6a3): Santa Cruz, Dallas, TX; lactate dehydrogenase A (LDHA), or HIF-1α: Abcam, Cambridge, MA; macrophage: ionized calcium-binding adapter molecule 1 (IBA-1), a marker exclusively for tissue macrophages, Abcam; and CD3: a marker for lymphocytes, Abcam.

Techniques: Imaging, Expressing, Immunohistochemical staining, Staining, Control, Infection

Journal: Pathogens

Article Title: Genotyping, Assessment of Virulence and Antibacterial Resistance of the Rostov Strain of Mycobacterium tuberculosis Attributed to the Central Asia Outbreak Clade

doi: 10.3390/pathogens9050335

Figure Lengend Snippet: MIC distribution of the Rostov strain of Mycobacterium tuberculosis for antibiotics and drug resistance markers.

Article Snippet: The M. tuberculosis bacillary burden in lungs and livers was counted by homogenates plating onto Middlebrook 7H11 agar.

Techniques: Marker

Journal: Pathogens

Article Title: Genotyping, Assessment of Virulence and Antibacterial Resistance of the Rostov Strain of Mycobacterium tuberculosis Attributed to the Central Asia Outbreak Clade

doi: 10.3390/pathogens9050335

Figure Lengend Snippet: Growth of H37Rv and Rostov strains of M. tuberculosis in 7H9 broth. ( A )—Growth index (calculated by the colony-forming unit (CFU) at each time point divided by the CFU at initial time point); ( B )—Comparison of C max (a maximum point on the growth curve); *—values of p < 0.05.

Article Snippet: The M. tuberculosis bacillary burden in lungs and livers was counted by homogenates plating onto Middlebrook 7H11 agar.

Techniques: Comparison

Journal: Pathogens

Article Title: Genotyping, Assessment of Virulence and Antibacterial Resistance of the Rostov Strain of Mycobacterium tuberculosis Attributed to the Central Asia Outbreak Clade

doi: 10.3390/pathogens9050335

Figure Lengend Snippet: Comparison of the survival curves of C57BL/6 mice infected by M. tuberculosis strains. Data were analyzed by the Gehan–Breslow–Wilcoxon test. The value of p < 0.05 was taken as statistically significant.

Article Snippet: The M. tuberculosis bacillary burden in lungs and livers was counted by homogenates plating onto Middlebrook 7H11 agar.

Techniques: Comparison, Infection

Journal: Pathogens

Article Title: Genotyping, Assessment of Virulence and Antibacterial Resistance of the Rostov Strain of Mycobacterium tuberculosis Attributed to the Central Asia Outbreak Clade

doi: 10.3390/pathogens9050335

Figure Lengend Snippet: Comparison of the weight changes curves of C57BL/6 mice infected by M. tuberculosis strains. The value of p < 0.05 was taken as statistically significant.

Article Snippet: The M. tuberculosis bacillary burden in lungs and livers was counted by homogenates plating onto Middlebrook 7H11 agar.

Techniques: Comparison, Infection

Journal: Pathogens

Article Title: Genotyping, Assessment of Virulence and Antibacterial Resistance of the Rostov Strain of Mycobacterium tuberculosis Attributed to the Central Asia Outbreak Clade

doi: 10.3390/pathogens9050335

Figure Lengend Snippet: Comparative characterization of the mortality, animal appearance and morphological description of internal organs of C57Bl/6 mice infected by the H37Rv and Rostov strains of M. tuberculosis .

Article Snippet: The M. tuberculosis bacillary burden in lungs and livers was counted by homogenates plating onto Middlebrook 7H11 agar.

Techniques: Infection

Journal: Pathogens

Article Title: Genotyping, Assessment of Virulence and Antibacterial Resistance of the Rostov Strain of Mycobacterium tuberculosis Attributed to the Central Asia Outbreak Clade

doi: 10.3390/pathogens9050335

Figure Lengend Snippet: Histology of lungs and livers of C57Bl/6 mice on the 30th day after intravenous inoculation by the M. tuberculosis strains H37Rv ( A —lungs, C —liver) and Rostov ( B —lungs, D —liver). 1, 2, 3 and 4—×4, ×10, ×20 and ×40 magnification, respectively. The arrow indicates the specific mice cells ( A 4, B 4—macrophages; C 4—lymphocytes; D 4—polymorphonuclear leukocytes).

Article Snippet: The M. tuberculosis bacillary burden in lungs and livers was counted by homogenates plating onto Middlebrook 7H11 agar.

Techniques:

Journal: Pathogens

Article Title: Genotyping, Assessment of Virulence and Antibacterial Resistance of the Rostov Strain of Mycobacterium tuberculosis Attributed to the Central Asia Outbreak Clade

doi: 10.3390/pathogens9050335

Figure Lengend Snippet: M. tuberculosis cells loads of the C57Bl/6 mice lungs and liver on the 30th day after the inoculation of bacteria; *—values of p ≤ 0.05; **—values of p ≤ 0.01.

Article Snippet: The M. tuberculosis bacillary burden in lungs and livers was counted by homogenates plating onto Middlebrook 7H11 agar.

Techniques: Bacteria

Journal: BMC Genomics

Article Title: Molecular epidemiology and transmission dynamics of multi-drug resistant tuberculosis strains using whole genome sequencing in the Amhara region, Ethiopia

doi: 10.1186/s12864-023-09502-2

Figure Lengend Snippet: Minimum spanning tree for 41 M. tuberculosis isolates based upon cgMLST analysis. M. tuberculosis cgMLST complex type / cluster-alert distance were 12 alleles. The numbers displayed on lines between circles represent the numbers of different alleles between different samples. Samples were grouped by color according to their cgMLST complex type (right side)

Article Snippet: Each isolate sequence was aligned to the Ridom SeqSphere M. tuberculosis cgMLST scheme, previously defined consisting of 2891 core genes (GenBank accession number NC_000962.3), for alignment and subsequent genomic analysis [ , ].

Techniques: